3N38
Ribonucleotide Reductase NrdF from Escherichia coli Soaked with Ferrous Ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2010-02-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.03324 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 79.137, 79.137, 267.981 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 68.530 - 1.900 |
| R-factor | 0.197 |
| Rwork | 0.196 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3n37 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.004 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 68.530 |
| High resolution limit [Å] | 1.900 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.6 | 25% PEG 4000, 0.1 M HEPES pH 7.6, 0.1 M lithium sulfate |
