3N37
Ribonucleotide Reductase Dimanganese(II)-NrdF from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2009-11-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 78.142, 78.142, 266.754 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 67.670 - 1.650 |
| R-factor | 0.168 |
| Rwork | 0.167 |
| R-free | 0.18500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r2f |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.088 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 67.670 |
| High resolution limit [Å] | 1.650 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M HEPES pH 7.5, 30% PEG 4000 |
