3N36

Erythrina corallodendron lectin mutant (Y106G) in complex with Galactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-04-10
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54
Spacegroup name	P 31 2 1
Unit cell lengths	102.584, 102.584, 57.112
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.610 - 2.300
R-factor	0.177
Rwork	0.177
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1sfy
RMSD bond length	0.008
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.137	0.444
Number of reflections	15677
<I/σ(I)>	16.71	5.5
Completeness [%]	99.8	100
Redundancy	8.2	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	20% PEG 3350, 0.25M diammonium hydrogen citrate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K