3N31
Crystal Structure of the complex of type I ribosome inactivating protein with fucose at 2.1A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2008-10-28 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8148 |
| Spacegroup name | H 3 |
| Unit cell lengths | 129.153, 129.153, 39.366 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 64.580 - 2.110 |
| R-factor | 0.17638 |
| Rwork | 0.174 |
| R-free | 0.22627 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aha |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.228 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.580 | 2.220 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 14192 | |
| <I/σ(I)> | 14.2 | 4.7 |
| Completeness [%] | 97.3 | 92.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 298 | 14% PEG6000, 0.1M Sodium Phosphate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
