3N2Q
Crystal structure of Sex pheromone staph-cAM373 precursor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 65.062, 65.062, 142.903 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.550 |
| R-factor | 0.21772 |
| Rwork | 0.215 |
| R-free | 0.27274 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.254 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.102 | 0.479 |
| Number of reflections | 11892 | |
| <I/σ(I)> | 46.9 | 6.2 |
| Completeness [%] | 99.1 | 100 |
| Redundancy | 9.5 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 297 | 2.1M DL-Malic acid, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
