3N2O
X-ray crystal structure of arginine decarboxylase complexed with Arginine from Vibrio vulnificus
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97935 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 101.648, 119.365, 121.840 |
| Unit cell angles | 90.00, 96.30, 90.00 |
Refinement procedure
| Resolution | 121.100 - 2.300 |
| R-factor | 0.18182 |
| Rwork | 0.179 |
| R-free | 0.23921 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.831 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 121.100 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.127 | 0.888 |
| Number of reflections | 127349 | |
| <I/σ(I)> | 17.4 | 2.1 |
| Completeness [%] | 98.8 | 100 |
| Redundancy | 4.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M HEPES pH 7.0, 0.2 M MgCl2, 12% PEG 4000., VAPOR DIFFUSION, HANGING DROP, temperature 293K |
