3N2K
TUBULIN-NSC 613862: RB3 Stathmin-like domain complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-10 |
| Detector | ADSC |
| Spacegroup name | P 65 |
| Unit cell lengths | 326.321, 326.321, 54.057 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 4.000 |
| R-factor | 0.22461 |
| Rwork | 0.223 |
| R-free | 0.25012 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sa0 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.795 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 4.100 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Number of reflections | 28503 | |
| <I/σ(I)> | 20 | 2.6 |
| Completeness [%] | 99.6 | 99.7 |
| Redundancy | 4 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | PEG, PIPES BUFFER, PH 7.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 277K |
