3N24
The crystal structure of a two-component sensor domain (3rd form) from Pseudomonas aeruginosa PA01
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.003, 61.093, 71.600 |
| Unit cell angles | 85.60, 70.47, 89.50 |
Refinement procedure
| Resolution | 36.630 - 2.056 |
| R-factor | 0.2064 |
| Rwork | 0.203 |
| R-free | 0.28160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kkb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.022 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.081 | 0.549 |
| Number of reflections | 46030 | |
| <I/σ(I)> | 14.4 | 1.54 |
| Completeness [%] | 97.2 | 90 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 0.2M sodium, 0.1M phosphate citrate, 20% PEG8000, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
