3N1W

Human FPPS COMPLEX WITH FBS_02

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2004-05-07
Detector	MARCCD165
Wavelength(s)	0.97933
Spacegroup name	P 41 21 2
Unit cell lengths	111.332, 111.332, 76.248
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.360 - 2.560
R-factor	0.213
Rwork	0.208
R-free	0.25700
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.007
RMSD bond angle	1.200
Data scaling software	XSCALE
Phasing software	APRV
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000		2.640
High resolution limit [Å]	2.560	13.300	2.560
Rmerge	0.062	0.021	0.348
Rmeas		0.020	0.380
Number of reflections	15908	120	1374
<I/σ(I)>	18.97	40.4	6.1
Completeness [%]	99.6	81.6	99.9
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.3	292	1.2M sodium potassium phosphate, 25% glycerol, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 292K