3N1R
Crystal Structure of ShhN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | RIGAKU SATURN 944+ |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 63.006, 63.006, 91.102 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.282 - 2.130 |
| R-factor | 0.2378 |
| Rwork | 0.235 |
| R-free | 0.28320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.939 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.130 |
| Number of reflections | 11846 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 298 | 20% Peg 3350, 200 mM NaF, 100 mM Tris, pH 8.0, VAPOR DIFFUSION, temperature 298K |
