3N1O

Crystal structure of IhhN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Detector	RIGAKU SATURN 944+
Spacegroup name	P 43 21 2
Unit cell lengths	67.965, 67.965, 243.281
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.990 - 2.550
R-factor	0.1995
Rwork	0.196
R-free	0.26050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.851
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.550
Number of reflections	18164

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	298	1M NaCitrate, 100mM Cacodylate, pH 6.0, VAPOR DIFFUSION, temperature 298K