3N1L
Crystal Structure of a StWhy2-rcERE32 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-04 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.10 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 166.667, 166.667, 166.667 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.667 - 2.350 |
| R-factor | 0.2221 |
| Rwork | 0.217 |
| R-free | 0.26260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l3a |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.321 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 8716 | |
| <I/σ(I)> | 16.5 | 2.3 |
| Completeness [%] | 99.5 | 95.4 |
| Redundancy | 29.1 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 21% PEG6000, 0.1M Tris, 2.0M LiCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
