3N1K

Crystal Structure of a StWhy2-cERE32 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-04
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.10
Spacegroup name	F 4 3 2
Unit cell lengths	166.934, 166.934, 166.934
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.734 - 2.702
R-factor	0.2129
Rwork	0.207
R-free	0.25710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l3a
RMSD bond length	0.006
RMSD bond angle	0.953
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	5865
<I/σ(I)>	12.7	2.2
Completeness [%]	99.6	96
Redundancy	30.5	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	19% PEG6000, 0.1M Tris, 1.5M LiCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K