3N1I
Crystal Structure of a StWhy2-ERE32 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.08 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 167.164, 167.164, 167.164 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.171 - 2.200 |
| R-factor | 0.2295 |
| Rwork | 0.227 |
| R-free | 0.26180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l3a |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.099 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 10665 | |
| <I/σ(I)> | 23.1 | 3.8 |
| Completeness [%] | 99.8 | 98.7 |
| Redundancy | 35.9 | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 13.5% PEG6000, 0.1M Tris, 1.0M LiCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
