3N0I
Crystal Structure of Ad37 fiber knob in complex with GD1a oligosaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.990, 69.770, 74.900 |
| Unit cell angles | 90.00, 94.92, 90.00 |
Refinement procedure
| Resolution | 32.900 - 1.650 |
| R-factor | 0.16 |
| Rwork | 0.160 |
| R-free | 0.18700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uxe |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.502 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.900 | 1.690 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.051 | 0.540 |
| Number of reflections | 74636 | |
| <I/σ(I)> | 14.2 | 2.2 |
| Completeness [%] | 99.1 | 98.4 |
| Redundancy | 4.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 293 | 26% PEG 8000, 0.05M zinc acetate, 0.1M HEPES, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
