3N0D
Crystal structure of WDR5 mutant (W330F)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002+ |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-31 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.717, 79.654, 45.967 |
| Unit cell angles | 90.00, 101.73, 90.00 |
Refinement procedure
| Resolution | 31.850 - 2.300 |
| R-factor | 0.17027 |
| Rwork | 0.167 |
| R-free | 0.23307 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h14 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.672 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.850 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.079 | 0.218 |
| Total number of observations | 67971 | |
| Number of reflections | 19383 | |
| Completeness [%] | 99.8 | 98.2 |
| Redundancy | 3.51 | 2.82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 18% PEG 3350, 0.1M Hepes, 14mM (NH4)2SO4, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
