3N08
Crystal Structure of a Putative PhosphatidylEthanolamine-Binding Protein (PEBP) Homolog CT736 from Chlamydia trachomatis D/UW-3/CX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-12 |
| Detector | RAYONIX MX-300 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.115, 37.847, 55.101 |
| Unit cell angles | 105.44, 96.50, 108.36 |
Refinement procedure
| Resolution | 26.190 - 1.250 |
| R-factor | 0.153 |
| Rwork | 0.152 |
| R-free | 0.17300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fux |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.373 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_348)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.270 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.080 | 0.607 |
| Number of reflections | 70466 | |
| <I/σ(I)> | 14.75 | 2.42 |
| Completeness [%] | 94.9 | 92.3 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 298 | 20%PEG 3350,0.2M Ca Chloride , pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
