3MZY

The Crystal Structure of the RNA polymerase sigma-H factor from Fusobacterium nucleatum to 2.5A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-21
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9794
Spacegroup name	P 65 2 2
Unit cell lengths	71.761, 71.761, 153.623
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.070 - 2.500
R-factor	0.236
Rwork	0.234
R-free	0.26900
RMSD bond length	0.011
RMSD bond angle	1.138
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.590
High resolution limit [Å]	2.500	5.380	2.500
Rmerge	0.083	0.057	0.599
Number of reflections	8730
<I/σ(I)>	9
Completeness [%]	99.9	99.4	100
Redundancy	14.4	13.6	14.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	20% PEG 1000, 0.1M Tris pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K