3MZV
Crystal structure of a decaprenyl diphosphate synthase from Rhodobacter capsulatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-26 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.459, 90.245, 80.167 |
| Unit cell angles | 90.00, 125.76, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.194 |
| Rwork | 0.193 |
| R-free | 0.22100 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.507 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.064 | 0.479 |
| Number of reflections | 56604 | |
| <I/σ(I)> | 13 | 3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.1 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 294 | 100mM MES pH 6.5, 30% PEG MME 5K, 200mM ammonium sulfate, vapor diffusion, temperature 294K |
