3MZR
RNase crystals grown in loops/micromounts
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-10 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 64.190, 64.190, 63.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.500 |
| R-factor | 0.17709 |
| Rwork | 0.176 |
| R-free | 0.19256 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 3DH5 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.766 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.081 | 0.867 |
| Number of reflections | 24805 | |
| <I/σ(I)> | 16 | 2.4 |
| Completeness [%] | 99.9 | |
| Redundancy | 6 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Crystals grown in loops | 5.5 | 295 | 1.7M Ammonium sulfate, 2M NaCal, 0.1M Sodium acetate, pH 5.5, Crystals grown in loops, temperature 295K |
