3MZK

Sec13/Sec16 complex, S.cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	56.734, 139.012, 205.424
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.713 - 2.690
R-factor	0.2002
Rwork	0.198
R-free	0.24340
Structure solution method	SAD
Starting model (for MR)	PARTIAL MODEL FROM SEMET CRYSTAL
RMSD bond length	0.005
RMSD bond angle	0.836
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.740
High resolution limit [Å]	2.650	2.650
Rmerge	0.074	0.653
Number of reflections	45333
<I/σ(I)>	20.7	1.9
Completeness [%]	99.8	99.4
Redundancy	3.7	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	0.1mM bis-Tris propane, 0.2M NaBr, 12% polyethylene glycol 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K