3MZ1
The crystal structure of a possible TRANSCRIPTION REGULATOR PROTEIN from Sinorhizobium meliloti 1021
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.345, 83.325, 85.067 |
| Unit cell angles | 90.00, 91.65, 90.00 |
Refinement procedure
| Resolution | 36.422 - 1.880 |
| R-factor | 0.1865 |
| Rwork | 0.184 |
| R-free | 0.22410 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.166 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 1.910 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.111 | 0.841 |
| Number of reflections | 75983 | |
| <I/σ(I)> | 23.1 | 1.8 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 4.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M MES, 0.2M Ammonium sulfate, 30% PEG5Kmme, 1/10V8, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
