3MYU

Mycoplasma genitalium MG289

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-03
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	49.279, 90.366, 175.363
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.180 - 1.950
R-factor	0.2222
Rwork	0.221
R-free	0.25530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3eki
RMSD bond length	0.006
RMSD bond angle	0.938
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX ((AUTOMR: 1.6.1_348))
Refinement software	PHENIX ((phenix.refine: 1.6.1_348))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.180	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.076	0.560
Number of reflections	52800
<I/σ(I)>	22	2.7
Completeness [%]	90.9	90
Redundancy	7.2	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.6	298	0.2 M AMMONIUM SULFATE, 0.1 M SODIUM ACETATE TRIHYDRATE pH 4.6 and 15% PEG 4000 AND HAMPTON SILVER BULLET BIO CONDITION 49, VAPOR DIFFUSION, HANGING DROP, temperature 298K