3MYO
Crystal structure of tagatose-1,6-bisphosphate aldolase from Streptococcus pyogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6C1 |
| Synchrotron site | PAL/PLS |
| Beamline | 6C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-20 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.2399 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 126.149, 126.149, 148.701 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.980 - 2.500 |
| R-factor | 0.20136 |
| Rwork | 0.200 |
| R-free | 0.22570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3myp |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.152 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.096 | 0.336 |
| Number of reflections | 42128 | |
| <I/σ(I)> | 23.7 | 6.1 |
| Completeness [%] | 100 | |
| Redundancy | 8.1 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 287 | 0.1M sodium citrate, 0.1M MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 287K |
