3MYJ
Human Class I MHC HLA-A2 in complex with the WT-1 (126-134) (R1Y) peptide variant.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.202, 62.826, 74.755 |
| Unit cell angles | 81.91, 75.90, 77.94 |
Refinement procedure
| Resolution | 20.000 - 1.890 |
| R-factor | 0.188 |
| Rwork | 0.185 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tvb |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.877 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.930 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.063 | 0.419 |
| Number of reflections | 66006 | |
| <I/σ(I)> | 14.5 | 2 |
| Completeness [%] | 96.9 | 95.9 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | PEG3350 24%, MES 0.025M, KCl 0.1M, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
