3MYD
Structure of the Cytoplasmic domain of FlhA from Helicobacter pylori
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2009-09-24 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97934, 0.97882, 0.97907, 0.98166 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 69.193, 136.200, 107.682 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.650 - 2.400 |
R-factor | 0.22557 |
Rwork | 0.224 |
R-free | 0.26470 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.225 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.074 | 0.386 |
Number of reflections | 20380 | |
<I/σ(I)> | 29.8 | 3.7 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 7.3 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | precipitant: 15% MME-PEG 5K, 100 mM Na Cacodylate pH 6.2-6.7, 0.2 M Ammonium Sulfate, 6-8% v/v isopropanol,, VAPOR DIFFUSION, HANGING DROP, temperature 298K |