3MYD
Structure of the Cytoplasmic domain of FlhA from Helicobacter pylori
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2009-09-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97934, 0.97882, 0.97907, 0.98166 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 69.193, 136.200, 107.682 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.650 - 2.400 |
| R-factor | 0.22557 |
| Rwork | 0.224 |
| R-free | 0.26470 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.225 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.074 | 0.386 |
| Number of reflections | 20380 | |
| <I/σ(I)> | 29.8 | 3.7 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.3 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | precipitant: 15% MME-PEG 5K, 100 mM Na Cacodylate pH 6.2-6.7, 0.2 M Ammonium Sulfate, 6-8% v/v isopropanol,, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
