3MXZ

Crystal Structure of tubulin folding cofactor A from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-10-13
Detector	MAR CCD 165 mm
Wavelength(s)	0.80150
Spacegroup name	I 41
Unit cell lengths	54.974, 54.974, 67.419
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	17.683 - 1.599
R-factor	0.177
Rwork	0.176
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qsd
RMSD bond length	0.007
RMSD bond angle	1.064
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.660
High resolution limit [Å]	1.599	1.600
Rmerge		0.330
Number of reflections	13258
<I/σ(I)>		4.24
Completeness [%]	99.8	98.5
Redundancy		5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	289	35% PEG3350, 0.4M Sodium Nitrate, 0.1M Sodium Acetate, pH 4.6, vapor diffusion, sitting drop, temperature 289K