3MXU
Crystal structure of glycine cleavage system protein H from Bartonella henselae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-06 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | H 3 2 |
Unit cell lengths | 98.910, 98.910, 131.540 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.893 - 1.800 |
R-factor | 0.177 |
Rwork | 0.176 |
R-free | 0.19600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hgb |
RMSD bond length | 0.012 |
RMSD bond angle | 1.349 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.1.4) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.900 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.050 | 0.557 |
Number of reflections | 23036 | 1664 |
<I/σ(I)> | 20.95 | 2.2 |
Completeness [%] | 99.5 | 98.9 |
Redundancy | 5 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 289 | 0.1 M sodium citrate pH 4.0, 0.8 M ammonium citrate, 25% ethylene glycol as cryo-protectant, VAPOR DIFFUSION, SITTING DROP, temperature 289K |