3MXJ

Crystal Structure of the mTREX1 Apoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	64.160, 85.720, 100.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.781 - 1.950
R-factor	0.2055
Rwork	0.204
R-free	0.23710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.050
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.4_113))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.000	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.057	0.276
Number of reflections	40918
<I/σ(I)>	16	4.1
Completeness [%]	99.7	97.1
Redundancy	6.72	4.63

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.25 M tri-sodium citrate dihydrate 20% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K