3MXC
Structures of Grb2-SH2 Domain and AICD peptide Complexes Reveal a Conformational Switch and Their Functional Implications.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-07-02 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 59.369, 59.369, 117.101 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.690 - 2.000 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jyr |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.600 |
| Data reduction software | AUTOMAR |
| Phasing software | AMoRE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.090 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.042 | 0.270 |
| Number of reflections | 8497 | |
| <I/σ(I)> | 5.9 | 2.1 |
| Completeness [%] | 95.3 | 95 |
| Redundancy | 5.48 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 293 | 0.1 M Tris ,0.1M Bicine, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
