3MXB
Molecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.55 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.050, 89.360, 86.340 |
| Unit cell angles | 90.00, 99.51, 90.00 |
Refinement procedure
| Resolution | 35.840 - 2.300 |
| R-factor | 0.1825 |
| Rwork | 0.180 |
| R-free | 0.23120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.233 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.790 | 2.300 |
| High resolution limit [Å] | 2.190 | 2.200 |
| Rmerge | 0.072 | 0.482 |
| Number of reflections | 50179 | |
| <I/σ(I)> | 8.8 | 2.3 |
| Completeness [%] | 97.7 | 93.2 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 298 | 0.26M ammonium phosphate monobasic, 35%(v/v) glycerol, pH 7.0, VAPOR DIFFUSION, temperature 298K |
