3MX0
Crystal Structure of EphA2 ectodomain in complex with ephrin-A5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.98792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.859, 89.049, 198.150 |
Unit cell angles | 90.00, 96.22, 90.00 |
Refinement procedure
Resolution | 45.028 - 3.506 |
R-factor | 0.2369 |
Rwork | 0.234 |
R-free | 0.29510 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.472 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.630 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.104 | 0.219 |
Number of reflections | 23143 | |
<I/σ(I)> | 8.8 | 6.2 |
Completeness [%] | 91.4 | 71.1 |
Redundancy | 5.4 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 298 | 20% PEG 3350, 200 mM Ammonium Citrate, 100 mM Na Citrate, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |