Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-22 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9782 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.981, 41.057, 73.586 |
| Unit cell angles | 90.00, 109.33, 90.00 |
Refinement procedure
| Resolution | 18.279 - 1.400 |
| R-factor | 0.167 |
| Rwork | 0.166 |
| R-free | 0.18400 |
| Starting model (for MR) | 3KS1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.257 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX (1.6.1_348) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.064 | 0.194 |
| Number of reflections | 90618 | |
| <I/σ(I)> | 15.1 | 6.2 |
| Completeness [%] | 96.8 | 95.5 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | 50 mM Tris, 1.2 M sodium citrate, 5 ul + 5 ul droplet, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
