3MWN
Structure of the Novel 14 kDa Fragment of alpha-Subunit of Phycoerythrin from the Starving Cyanobacterium Phormidium Tenue
Replaces: 2G9MExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-12-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5414 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.357, 83.789, 62.484 |
| Unit cell angles | 90.00, 90.17, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.600 |
| R-factor | 0.238 |
| Rwork | 0.238 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lia |
| RMSD bond length | 0.025 |
| RMSD bond angle | 0.030 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 18133 | |
| <I/σ(I)> | 5 | 2 |
| Completeness [%] | 85.0 | 92.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 50MM SODIUM CACODYLATE, 20% PEG 2000, 30MM AMMONIUM SULFATE, pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
