3MWM

Graded expression of zinc-responsive genes through two regulatory zinc-binding sites in Zur

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-24
Detector	ADSC QUANTUM 270
Wavelength(s)	1.28286
Spacegroup name	C 1 2 1
Unit cell lengths	103.599, 36.978, 71.645
Unit cell angles	90.00, 103.26, 90.00

Refinement procedure

Resolution	26.020 - 2.400
R-factor	0.206
Rwork	0.206
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3eyy
RMSD bond length	0.008
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CCP4
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.400	2.400
Number of reflections	9706
Completeness [%]	90.0	85.7
Redundancy	3.7	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	micro bath	6.5	296	21% (w/v) polyethylene glycol 3350, 0.1M Bis-Tris pH 6.5, 0.1M Magnesium formate, 80mM 3-[(3-Cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonate (CHAPSO), micro bath, temperature 296K