3MWM
Graded expression of zinc-responsive genes through two regulatory zinc-binding sites in Zur
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-24 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.28286 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.599, 36.978, 71.645 |
| Unit cell angles | 90.00, 103.26, 90.00 |
Refinement procedure
| Resolution | 26.020 - 2.400 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eyy |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CCP4 |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 9706 | |
| Completeness [%] | 90.0 | 85.7 |
| Redundancy | 3.7 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | micro bath | 6.5 | 296 | 21% (w/v) polyethylene glycol 3350, 0.1M Bis-Tris pH 6.5, 0.1M Magnesium formate, 80mM 3-[(3-Cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonate (CHAPSO), micro bath, temperature 296K |
