3MWJ

Q28E mutant of HERA N-terminal RecA-like domain, apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-04-23
Detector	PSI PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	91.541, 91.541, 108.116
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.290 - 1.400
R-factor	0.14295
Rwork	0.141
R-free	0.17618
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gxs
RMSD bond length	0.018
RMSD bond angle	1.723
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.6.0070)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.300	1.420
High resolution limit [Å]	1.400	1.400
Rmerge	0.021	0.417
Number of reflections	90708
<I/σ(I)>	15	1.9
Completeness [%]	100.0	100
Redundancy	20.2	20.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1M Bis-Tris pH 6.5, 28% MPEG 2000, VAPOR DIFFUSION, SITTING DROP, temperature 293K