3MWH
The 1.4 Ang crystal structure of the ArsD arsenic metallochaperone provides insights into its interactions with the ArsA ATPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-07-13 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.676, 74.223, 40.830 |
| Unit cell angles | 90.00, 97.77, 90.00 |
Refinement procedure
| Resolution | 40.460 - 2.050 |
| R-factor | 0.27351 |
| Rwork | 0.271 |
| R-free | 0.32419 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kgk |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.992 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.460 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.348 | |
| Number of reflections | 13712 | |
| Completeness [%] | 97.8 | 98.6 |
| Redundancy | 3.12 | 3.05 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 25% PEG 6000, 0.1M Calcium acetate, 0.1M HEPES, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
