3MW2
Crystal structure of beta-neurexin 1 with the splice insert 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Detector technology | CCD |
| Collection date | 2009-06-12 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 85.890, 59.819, 105.697 |
| Unit cell angles | 90.00, 103.86, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.690 |
| R-factor | 0.21016 |
| Rwork | 0.206 |
| R-free | 0.28724 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 3BOD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.234 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.700 |
| Rmerge | 0.090 |
| Number of reflections | 14339 |
| <I/σ(I)> | 19.7 |
| Completeness [%] | 78.3 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.9 | 293 | 26% PEG1000, 0.2M lithium sulfate, 0.1M phosphate-citrate, pH 4.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
