3MVX
X-ray structure of the reduced NikA/1 hybrid, NikA/1-Red
Experimental procedure
Experimental method | 0.9796 |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Detector technology | CCD |
Collection date | 2008-11-28 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 86.967, 94.616, 125.025 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.300 - 1.700 |
R-factor | 0.16601 |
Rwork | 0.164 |
R-free | 0.20374 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zlq |
RMSD bond length | 0.028 |
RMSD bond angle | 2.256 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.300 | |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 110957 | |
<I/σ(I)> | 22.63 | 5.9 |
Completeness [%] | 97.0 | 93 |
Redundancy | 7 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 293 | 1.8M ammonium sulfate, 0.1M sodium acetate, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |