3MVG
Native structure of IRIP, a type I ribosome inactivating protein from Iris hollandica var. at 1.25 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-03 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.81480 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.879, 84.809, 69.080 |
| Unit cell angles | 90.00, 97.67, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.250 |
| R-factor | 0.16931 |
| Rwork | 0.168 |
| R-free | 0.18542 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m2t |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.185 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.280 |
| High resolution limit [Å] | 1.250 | 1.247 |
| Rmerge | 0.051 | 0.649 |
| Total number of observations | 1839695 | |
| Number of reflections | 157596 | |
| <I/σ(I)> | 25.6 | 2.67 |
| Completeness [%] | 99.8 | 98 |
| Redundancy | 4.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 1.9M ammonium sulfate in 20% w/v glycerol, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
