3MVF
Crystal Structure of Nitrophorin 4 from Rhodnius prolixus Complexed with Nitrite at pH 7.4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91892 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.301, 42.909, 52.460 |
| Unit cell angles | 90.00, 94.05, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.400 |
| R-factor | 0.13889 |
| Rwork | 0.136 |
| R-free | 0.18717 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.219 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.530 | 1.440 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.038 | 0.193 |
| Number of reflections | 30694 | |
| Completeness [%] | 97.7 | 93.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 297 | Ammonium phosphate, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
