3MVE
Crystal structure of a novel pyruvate decarboxylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-08 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.99999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.834, 88.201, 79.629 |
| Unit cell angles | 90.00, 106.62, 90.00 |
Refinement procedure
| Resolution | 48.630 - 2.200 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.220 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Number of reflections | 41404 | |
| Completeness [%] | 84.4 | |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | micro bath | 8.5 | 295 | 1.8M am-sulfate, 0.1M tris-HCl (pH 8.5), 0.2M na-acetate, micro bath, temperature 295K |
