3MVC
High resolution crystal structure of the heme domain of GLB-6 from C. elegans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2009-12-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.1158 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.582, 76.347, 62.696 |
Unit cell angles | 90.00, 91.76, 90.00 |
Refinement procedure
Resolution | 33.705 - 1.401 |
R-factor | 0.1629 |
Rwork | 0.161 |
R-free | 0.20500 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.854 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.420 |
High resolution limit [Å] | 1.400 | 1.400 |
Number of reflections | 130779 | |
<I/σ(I)> | 21.5 | |
Completeness [%] | 98.3 | 95.1 |
Redundancy | 4.2 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 1.0 M NaNO3 and 0.1 M Na(CH3COO) pH 4.5) and 1 ul of 10 mM praseodymium (III) acetate solution, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 1.0 M NaNO3 and 0.1 M Na(CH3COO) pH 4.5) and 1 ul of 10 mM praseodymium (III) acetate solution, VAPOR DIFFUSION, SITTING DROP, temperature 293K |