3MV2
Crystal Structure of a-COP in Complex with e-COP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03316 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 329.127, 74.382, 97.247 |
| Unit cell angles | 90.00, 102.27, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.900 |
| Rwork | 0.246 |
| R-free | 0.29700 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.640 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 51235 | |
| <I/σ(I)> | 1.7 | |
| Completeness [%] | 93.3 | |
| Redundancy | 3.1 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 100 mM HEPES, pH. 7.2 200 mM ammonium citrate 18 (w/v) % PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
