3MUQ
The crystal structure of a conserved functionally unknown protein from Vibrio parahaemolyticus RIMD 2210633
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97901 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 107.075, 109.088, 51.665 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.509 - 2.053 |
| R-factor | 0.1663 |
| Rwork | 0.164 |
| R-free | 0.20660 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.052 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.139 | 0.778 |
| Number of reflections | 38552 | |
| <I/σ(I)> | 13.3 | 1.88 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.9 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 2.1M DL-malic acid, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
