3MUI
Enzyme-Substrate interactions of IXT6, the intracellular xylanase of G. stearothermophilus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-05 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.939 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 169.270, 79.859, 78.820 |
| Unit cell angles | 90.00, 91.47, 90.00 |
Refinement procedure
| Resolution | 19.970 - 1.800 |
| Rwork | 0.195 |
| R-free | 0.21300 |
| Starting model (for MR) | 2q8x |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.970 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 91101 | |
| <I/σ(I)> | 15.2 | 2.12 |
| Completeness [%] | 93.9 | 81.9 |
| Redundancy | 2.98 | 2.74 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M sodium cacodylate pH 6.5, 1.9M soduim acetate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
