3MTW

Crystal structure of L-Lysine, L-Arginine carboxypeptidase Cc2672 from Caulobacter Crescentus CB15 complexed with N-methyl phosphonate derivative of L-Arginine

Replaces:  3GNH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-06
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97915
Spacegroup name	I 4 3 2
Unit cell lengths	200.315, 200.315, 200.315
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.354 - 1.700
R-factor	0.1813
Rwork	0.180
R-free	0.20310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.065
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	BALBES
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall
Low resolution limit [Å]	36.570
High resolution limit [Å]	1.700
Rmerge	0.067
Number of reflections	73648
Completeness [%]	98.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	2.0M ammonium sulfate, 0.1M Bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K