3MTV

The Crystal Structure of the PRRSV Nonstructural Protein Nsp1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-03-15
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000
Spacegroup name	P 41 21 2
Unit cell lengths	100.895, 100.895, 81.685
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.906 - 2.800
R-factor	0.2035
Rwork	0.201
R-free	0.26070
Structure solution method	MAD
RMSD bond length	0.038
RMSD bond angle	3.426
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.910	2.800
High resolution limit [Å]	2.800	2.800
Rmerge	0.097
Number of reflections	12021
Completeness [%]	99.0	99.6
Redundancy	18.1	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	100mM HEPES (pH 7.0), VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	6	291	100mM HEPES (pH 7.0), VAPOR DIFFUSION, HANGING DROP, temperature 291K