3MTT
Crystal structure of iSH2 domain of human p85beta, Northeast Structural Genomics Consortium Target HR5531C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.075 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 174.588, 174.588, 102.699 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.600 - 3.300 |
| R-factor | 0.2501 |
| Rwork | 0.249 |
| R-free | 0.27730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3L4Q chain D |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.625 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_377)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.600 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.088 | 0.567 |
| Number of reflections | 9161 | |
| <I/σ(I)> | 39 | 6.83 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 22.1 | 22.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 277 | 0.5 M Sodium Malonate, 0.1 M HEPES, , pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
