3MTR
Crystal structure of the Ig5-FN1 tandem of human NCAM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 77.090, 155.840, 71.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.18816 |
| Rwork | 0.183 |
| R-free | 0.23022 |
| Structure solution method | Molecular replacement and ARP |
| Starting model (for MR) | 2haz |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.567 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.900 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Number of reflections | 40353 | |
| <I/σ(I)> | 14.92 | 6.11 |
| Completeness [%] | 98.7 | 96.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 25% PEG 3350, 0.2 M ammonium sulfate, 0.1 M bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |
